OneStep 2X RT-qPCR Master Mix (Probe) 400µl (50 reactions)

Description: 
Virostem™ OneStep 2X RT-qPCR Master Mix is specially formulated for high-efficiency and fast reverse-transcription based quantitative-PCR (RT-qPCR) reactions. Our proprietary master mix contains Taq polymerase and MMLV enzymes, enhancer and stabilizer proteins, and other additives for highly specific and robust one-step amplification of RNA.
 
Compatibility: 
Virostem™ OneStep 2X RT-qPCR Master Mix is compatible with RNA samples isolated with standard column or precipitation-based purification kits and rapid nucleic acid extraction buffers such as VTMs/VNATs. 
For rapid nucleic acid extractions, we recommend using our specially formulated, nanodrop compatible Virolyse™ Cell Lysis Buffer (VLB-01), which is directly compatible with all Virostem PCR Master Mix products and can be used for one-step extraction of DNA, RNA and proteins directly from cells, saliva, nasal and nasopharyngeal samples in 10 minutes at room temperature and allows rapid cell to Ct detection of DNA and RNA.
 
Versatility: 
Our concentrated 2X formulation is highly flexible and provides 7.5µl of additional volume (in 15µl reactions) which can be used by the end-user for addition of primers, template and PCR dyes such as SYBR® and EvaGreen® or probes. Our unique buffer composition also enables highly specific amplifications without the need for extensive MgCl2 optimization.